服务热线
131 5529 7675
商品货号
CL0094
名称
bEnd.3 [BEND3] (小鼠脑微血管内皮细胞)
别称
bEND.3; b.End3; Bend.3; bEnd3; brain-derived Endothelial cells.3
种属
小鼠
年龄(性别)
6周龄
组织来源
大脑皮层
生长特性
贴壁细胞
细胞形态
上皮细胞样
背景描述
The cells were transformed by infection with the NTKmT retrovirus vector that expresses polyomavirus middle T antigen
生物安全等级
1
生长培养基
DMEM+10% FBS+1% P/S
推荐传代比例
1:4-1:6
推荐换液频率
2~3次/周
倍增时间
~24-32小时
冻存条件
冻存液:55%基础培养基 + 40% FBS + 5% DMSO;
温度:液氮
培养条件
气相:95%空气 + 5% CO2;
温度:37℃
抗原表达情况
ICAM-1 +; VCAM-1 +; MAdCAM-1 +
基因表达情况
von Willebrand factor, ICAM-1 +; VCAM-1 +; MAdCAM-1 +. The endothelial nature of these cells was confirmed by the observed expression of von Willebrand factor and uptake of fluorescently labeled low density lipoprotein (LDL). The expression of Peyer's Patch high endothelial receptor for lymphocytes, the mucosal vascular addressin (MAdCAM-1) and E-selectin can be induced on bEnd
保藏机构
ATCC; CRL-2299 BCRC; 60515 ECACC; 96091929
细胞运输
复苏后活细胞运输(T25瓶)或干冰冻存运输(1-2ml冻存管,+250元干冰费)