商品货号	CL0714
名称	SNU-387 (人肝癌细胞)
别称	SNU387; NCI-SNU-387
种属	人类
年龄（性别）	女性，41岁
组织来源	肝癌
生长特性	贴壁细胞
细胞形态	上皮细胞样
背景描述	SNU-387 was derived in 1990 by J.-G. Park and associates from a primary hepatocellular carcinoma taken from a Korean patient who had been treated by transcatheter arterial embolization with lipoidol plus a combination of doxorubicin and mitomycin-C. Tumor cells were initially cultured in ACL-4 medium supplemented with 5% heat-inactivated fetal bovine serum. After establishment, cultures were maintained in RPMI 1640 supplemented with 10% heat inactivated fetal bovine serum.
生物安全等级	2
生长培养基	RPMI-1640＋10% FBS＋1% P/S
推荐传代比例	1:3-1:6
推荐换液频率	2~3次/周
倍增时间	~61小时 (PubMed=7543080)
冻存条件	冻存液：55%基础培养基 + 40% FBS + 5% DMSO；或使用我司冻存液。 温度：液氮
培养条件	气相：95%空气 + 5% CO2； 温度：37℃
保藏机构	ATCC; CRL-2237
细胞运输	复苏后活细胞运输（T25瓶）或干冰冻存运输（1-2ml冻存管，+250元干冰费）