服务热线
131 5529 7675
商品货号
CL0707
名称
SNB-19 (人胶质瘤细胞)
别称
SNB.19; SNB19
年龄(性别)
男;75岁
组织来源
星形胶质瘤(U-251MG)
生长特性
贴壁细胞
细胞形态
成纤维细胞样
背景描述
Established from the surgical resection of a left parieto-occipital glioblastoma from a 47-year-old man in 1980; cells were described in the literature to secrete plasminogen activator, to be clonogenic in soft agar and to be tumorigenic in nude mice; DNA fingerprinting showed unequivocally that SNB-19 is a subclone of the astrocytoma cell line U-251 MG)。(STR检测位点同U-251MG)
生物安全等级
1
生长培养基
DMEM+10% FBS+1% P/S
推荐传代比例
1:4-1:6
推荐换液频率
2~3次/周
倍增时间
~24-36 hours
冻存条件
冻存液:55%基础培养基 + 40% FBS + 5% DMSO;或使用我司冻存液。
温度:液氮
培养条件
气相:95%空气 + 5% CO2;
温度:37℃
保藏机构
DSMZ; ACC-325
细胞运输
复苏后活细胞运输(T25瓶)或干冰冻存运输(1-2ml冻存管,+250元干冰费)